Written in English
Thesis (Ph.D.) -- University of Toronto, 2003.
|The Physical Object|
|Number of Pages||228|
The cis -oligomers of gp80 display rapid accumulation during cell aggregation, with a rate higher than that of the trans -dimers . Thus, gp80 cis- oligomers may be important precursors for trans- interactions. At sites of adhesion complex assembly, rafts can be amalgamated by receptor interactions , .Cited by: However, it is unclear how phospholipid-anchored adhesion molecules, such as the Dictyostelium receptor gp80, interact with the cytoskeleton. gp80 has been found to form adhesion complexes from. During development of Dictyostelium discoideum, a surface glycoprotein of M r 80, (gp80) is known to mediate EDTA-resistant cell-cell adhesion via homophilic interaction. Antibodies directed against a 13 amino acid sequence (mer) near the NH 2 terminus of the protein were found to inhibit cell reassociation. During development of Dictyostelium discoideum, a surface glycoprotein of Mr 80, (gp80) is known to mediate EDTA-resistant cell-cell adhesion via homophilic interaction. Antibodies directed against a 13 amino acid sequence (mer) near the NH2 terminus of the protein were found to inhibit cell reassociation. This mer also inhibited gpcell interaction and gpgp80 Cited by:
At the aggregation stage of Dictyostelium discoideum development, a cell surface glycoprotein of Mr 80, (gp80) has been found to mediate the EDTA-resistant type of cell-cell adhesion via. three forms of cell adhesion determine the life cycle ofDictyostelium: i) adhesion of bacteria to the surface of the growing amoebae, as the prerequisite for phagocytosis;ii) cell-substrate adhesion, necessary for both locomotion of the amoebae and migration of the slug;iii) cell-cell adhesion, essential for transition from the unicellular to the multicellular by: DdCAD-1 is a kD Ca 2+-dependent cell– cell adhesion molecule that is expressed soon after the initiation of development in Dictyostelium cells. DdCAD-1 is present on the cell surface as well as in the cytosol. However, the deduced amino acid sequence of DdCAD-1 lacks a hydrophobic signal peptide or any predicted transmembrane domain, suggesting that it may be presented on the cell Cited by: Although cell differentiation can occur in the absence of cell contact, regulated cell-cell adhesion is an important component of Dictyostelium morphogenesis, and a third adhesion molecule, gp
Beginning in and continuing to this day, Guenther Gerisch's work on the social ameba Dictyostelium discoideum has helped to make it the model organism of choice for studies of cellular activities that depend upon the actomyosin cytoskeleton. Gerisch has brought insight and quantitative rigor to cell biology by developing novel assays and by applying advanced Cited by: 4. Cell Growth and Development. Dictyostelium cells, including the wild-type axenic strain AX2 and the gpnull strain GT10 (36), were cultured either in association with Klebsiella aerogenes or axenically in HL5 liquid medium (44). Filipin staining revealed high sterol levels in both gpenriched cell-cell contacts and gp80 caps. Moreover, sterol sequestration by filipin and digitonin inhibited gpmediated cell-cell adhesion. gp is a membrane glycoprotein which has been implicated in cell–cell adhesion in the postaggregation stages of Dictyostelium development. An analysis of its tryptic peptides by mass spectrometry has identified gp as the product of the lagC gene, which was previously shown to play a role in morphogenesis and cell-type specification. Antibodies raised against the Cited by: